Antigen

Antigen Class

TFs and others

Antigen

Mlxipl

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

Liver

strain

C57BL/6

tissue

liver

genotype

liver specific ChREBPKO

treatment

PTU+T3

condition

hyperthyroidism

chip antibody

ChREBP (NB400-135 - Novus Biologicals)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with Anti-ChREBP Affinity-bound chromatin fragments were reverse-crosslinked, purified and used to construct DNA libraries for sequencing using the Illumina TruSeq ChIP Library Preparation Kit (Illumina)

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

41357625

Reads aligned (%)

97.5

Duplicates removed (%)

17.4

Number of peaks

433 (qval < 1E-05)

mm9

Number of total reads

41357625

Reads aligned (%)

97.3

Duplicates removed (%)

18.8

Number of peaks

438 (qval < 1E-05)