Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Mlxipl

Cell type

Cell type Class
Liver
Cell type
Liver
MeSH Description
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Attributes by original data submitter

Sample

source_name
Liver
strain
C57BL/6
tissue
liver
genotype
liver specific ChREBPKO
treatment
PTU+T3
condition
hyperthyroidism
chip antibody
ChREBP (NB400-135 - Novus Biologicals)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with Anti-ChREBP Affinity-bound chromatin fragments were reverse-crosslinked, purified and used to construct DNA libraries for sequencing using the Illumina TruSeq ChIP Library Preparation Kit (Illumina)

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
41357625
Reads aligned (%)
97.5
Duplicates removed (%)
17.4
Number of peaks
433 (qval < 1E-05)

mm9

Number of total reads
41357625
Reads aligned (%)
97.3
Duplicates removed (%)
18.8
Number of peaks
438 (qval < 1E-05)

Base call quality data from DBCLS SRA